OUT NOW our preprint titled “Massively parallel mapping of substrate cleavage sites defines dipeptidyl peptidase four subsite cooperativity”

Substrate specificity determines protease functions in physiology and in clinical and biotechnological application. However, affordable and unbiased assays for large-scale quantification of substrate cleavage have been lacking. Here, we develop hiMAPS (high-throughput mapping of protease cleavage sites), a cheap, mass spectrometry-based assay, to derive cleavage motifs for human Dipeptidyl Peptidase Four (DPP4), a key regulator of blood glucose levels.
Want to know more about it? Check out Rajani’s work – available as a preprint at bioRxiv