Author: grosshanslab

Substrate specificity determines protease functions in physiology and in clinical and biotechnological application. However, affordable and unbiased assays for large-scale quantification of substrate cleavage have been lacking. Here, we develop hiMAPS (high-throughput mapping of protease cleavage sites), a cheap, mass spectrometry-based assay, to derive cleavage motifs for human Dipeptidyl Peptidase Four (DPP4), a key regulator of blood glucose levels.
Want to know more about it? Check out Rajani’s work – available as a preprint at bioRxiv

UPDATE: Our preprint has been successfully published as a peer-reviewed paper in Mol Cell. Read more on the FMI webpage!